Agriculture Reference
In-Depth Information
Fig. 9.1   A flow chart showing the comparative study of both plant transformation systems-andro-
genesis and general transformation (seed-derived callus), in rice
and general transformation (seed-derived callus) are shown in Fig. 9.1 . Success of
plant transformation and its analysis in different stages is mentioned in the follow-
ing reports (Hiei et al. 1994 ; Hiei et al. 1997 ; Toki et al. 2006 ; Nishimura et al. 2006 ;
Lemaux PG 2008 ; Hiei and Komari 2008 ; Chauhan and Khurana 2010 ). General
steps of both the plant transformation systems are mentioned below accordingly.
2.2.1   Plasmid Construction of Targeted Genes
Plasmids are constructed harbouring the targated gene along the expression cassette
of plant promoters accompanied by selectable marker gene. Constructs are used
for anther/microspore-derived embryoids as explant for doubled haploid transgenic
plant production.
2.2.2   Transformation
Plant transformation is performed via Agrobacterium strains of LBA4404 for co-
cultivation using anther/microspore-derived embryos as explants. Transformation
is performed with plasmids containing targeted genes separately and for both con-
structs, the selectable marker is GUS.
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