An Overview of Omics for Wheat Grain Quality Improvement - Crop Improvement: New Approaches and Modern Techniques - page 325

Table 10.2 (continued)

Trait Locus Primer Allele Size (bp) PCR conditions Reference

GPC GPC-B1 F: AGCCAGGGATAGAGGAGGAA Gpc-B1 217 94 °C/30 s-58 °C/45 s-72 °C/40 s Uauy et al. 2006

R: AGCTGTGAGCTGGTGTCCTT
F: TCTCCAAGAGGGGAGAGACA

Gpc-B1 126 94 °C/30 s-58 °C/45 s-72 °C/40 s Distelfeld et al. 2006

R: TTCCTCTACCCATGAATCTAGCA

PPO
activity

Ppo-A1 F: AACTGCTGGCTCTTCTTCCCA Ppo-A1a 685 94 °C/60 s-65 °C/60 s-72 °C/60 s Sun et al. 2005

R: AAGAAGTTGCCCATGTCCGC Ppo-A1b 876

F: CCAGATACACAACTGCTGGC Ppo-A1a 290 95 °C/30 s-66-

54 °C/30 s-72 °C/60 s*

He et al. 2007

R: TGATCTTGAGGTTCTCGTCG Ppo-A1b 481

Ppo-D1 F: TGCTGACCGACCTTGACTCC Ppo-D1a 713

R: CTCGTCACCGTCACCCGTAT
F: TGAAGCTGCCGGTCATCTAC Ppo-D1b 490

R: AAGTTGCCCATGTCCTCGCC

Lipoxy-
genase
activity

TaLox-B1 F: CCATGACCTGATCCTTCCCTT TaLox-B1a 489 Geng et al. 2012

R: GCGCGGATAGGGGTGGT

F: ACGATGTGAGTTGTGACTTGTGA TaLox-B1b 791
R: GCGCGGATAGGGGTGC

Yellow
pigment
content

Psy-A1 F: GGACCTTGCTGATGACCGAG PsyA1a 194 95 °C/30 s-65 °C/30 s-72 °C/30 s He et al. 2008

R: TGACGGTCTGAAGTGAGAATGA PsyA1b 231

F: GCCAGCCCTTCAAGGACATG PsyA1a 1686 95 °C/60 s-60 °C/2 m-72 °C/30 s He et al. 2009a

R: CAGATGTCGCCACACTGCCA PsyA1c 1001

Psy-B1 F: GCCACAACTTGAATGTGAAAC Psy-B1a 151 95 °C/60 s-60 °C/2 m-72 °C/30 s He et al. 2009a

R: ACTTCTTCCATTTGAACCCC Psy-B1b 156

F: GCCACCCACTGATTACCACTA Psy-B1c 428

R: CCAAGGTGAGGGTCTTCAAC