Agriculture Reference
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that incorporation of Azolla-Anabaena to paddy field increases rice yields and ad-
dition of dried Azolla filiculoides at the rate of 93 kg N/ha has increased a rice yield
upto 70 %, the increase obtained with an equivalent amount of ammonium sulphate
(Anitha and Kannaiyan 1999 ). In a field experiment the cyanobacteria was used
to degrade coir pith with the help of lignolytic enzyme. (Malliga et al. 1996 ) and
produced cyanopith, it can be used as bio-fertilizer to improve the crop productivity
(Jha and Prasad 2005 ). Coir pith contains high lignin (31 %), cellulose (27 %), con-
tent (Bhat et al. 2003 ) and carbon nitrogen ratio (C/N) of 104:1 (Palaniappan 2005 ).
Manoharan et al. 2011 used cynopith as bio-fertilizers on Amaranthus dubius that
increases the growth of Amaranthus .
Azospirillum
Azospirillum is microaerophilic , free living, non-symbiotic, loosely associative
nitrogen fixing bacteria and it establishes a close association with various plants
mainly with C 4 maize, sorghum, sugarcane, ray grass, Amaranthus etc. This micro-
organism fixes atmospheric N and makes it available for plants in asymbiotic man-
ner (Steenhoudt and Vanderleyden 2006 ) . Azospirillum grows in the rhizosphere of
the plants or occasionally penetrates into the root tissues but is not able to produce
any visible nodule or out growth on the root tissue but grows intracellularly (Saikia
et al. 2007 ). This association is due to the ability of the microbe to use malic acid,
an organic acid formed for capturing CO 2 as a carbon source. It also secretes vari-
ous phytohormones which include gibberellins, cytokinins, auxins and affect de-
velopment and morphology of root by increasing root length, number of root hair
cells, lateral roots. Azosprillium also secretes iron-chelating siderphores that help
in the sequestering of iron sufficient for plant growth (Romerheld and Marshner
1986 ). A free living nitrogen fixing bacteria was for the first time reported by Bei-
jerinck in 1925 under the name of Spirillum lipoferum and later on renamed this
organism as Azospirillum (nitrogen fixing Spirillum) in 1978. Azosprillium is one
of recognized dominant soil microbe and is able to fix about 10-40 kgN/ha. The
Azosprillium inoculation improves vegetative growth of the plants (Naderifar and
Daneshian 2012 ). Till date only four species of Azospirillum have been identified
which include A. lipoferum, A. brasilense, A. amazonense, A. iraquense . Among
these species only A. brasilense and A. lipoferum are very common in Indian soils.
Inoculation of vegetable crops with Azospirillum has resulted in yield enhancement.
The field experiment of Azosprillium with maize was examined and was confirmed
that this association benefits enzyme activating glutamine synthetase and glutamine
synthetase in the leaves of paranodulated maize plants. Bhaskara Rao and Charyulu
( 2005 ) studied the association of A. lipoferum inoculated to foxtail millet plant in
combination with N fertilizer and demonstrated the increase in plant growth level,
dry weight of shoot and root over when compared with control plants.
Maize plants inoculated with Azospirillum showed high rate of photosynthesis
and stomatal conduction leading to high yield compared to control plants (Kumar
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