Chemistry Reference
In-Depth Information
R
N
N
-
+
N
O
S
O
H
N
R
-
+
+
Carbonic
anhydrase
N
His
His
Zn
His
N
+
N
3
R
N
H
2
N
37 °C for 40 h
S
panel of 20 azide
fragments
H
2
N
O
S
O
O
O
K
d
=
37
nM
11
in situ
hits
(
K
d
range 0.2
-
7
nM)
Scheme 7.7
In situ
Click chemistry targeting carbonic anhydrase.
Figure 7.17
Schematic representation of microfluidic chemical reaction circuit used for
parallel screening with
in situ
Click chemistry. Reproduced with permission from Wang,
J., Sui, G., Mocharla, V.P., Lin, R.J., Phelps, M.E., Kolb, H.C. and Tseng, H.-R., Integrated
microfluidics for parallel screening of an
in situ
click chemistry library.
Angewandte Chemie
International Edition
2006,
45
, 5276-5281. Copyright Wiley-VCHVerlag GmbH.
