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(a)
[CA II]
9-
[CA II]
10-
[CA II]
8-
2800
3600
m/z
3200
(b)
[CA II]
9-
[CA II-
1A-1E
]
9-
[CA II-
1
]
9-
2800
3200
3600
m
/z
(c)
[CA II]
8-
[
1E
-H]
-
[CA II]
9-
[
1C
-H]
-
[
1D
-H]
-
[
1A
-H]
-
[
1B
-H]
-
×20
400
450
m
/z
500
3000
3200
m
/z
3600
Figure 7.13
(a) ESI-FTMS negative ion mass spectrum of CA II (30
μ
M) in 10 mM NH
4
OAc
solution, 1% DMSO; (b) ESI-FTMS negative ion mass spectrum of a mixture of CA II (30
μ
M)
and DCL containing ten possible hydrazone products (
1A-E
,
2A-E
)in10mMNH
4
OAc,
1% DMSO; (c) tandem mass spectrum obtained following collision-activated dissociation
(CAD) of CA II-hydrazone noncovalent complexes to identify the hydrazones. Reprinted
from Poulsen S.-A., Direct screening of a dynamic combinatorial library using mass spec-
trometry.
Journal of the American Society for Mass Spectrometry
,
17
, 1074-1080. Copyright
(2006), with permission from Elsevier.
complexes with good sensitivity down to 5 mol% ligand, whereas at 1 mol% ligand and
below, the sensitivity of detection is at its limit and it is difficult to discern the protein-ligand
complex confidently in the presence of the free protein (unpublished work). Nanoelectro-
spray ionization (nanoESI), as described earlier in this chapter, has superior capabilities
