Biomedical Engineering Reference
In-Depth Information
Technology
Probe Assay sensitivity
detection
Assay description
Detection limits
sensitivity
(amount/copies)
(probes
/
µ
m
2
)
Fluorescently-
-
T: Total RNA
2.5
µ
g
labelled DNA
D: Laser scanning
dendrimers [21]
Up-converting
Single
T: Labelled DNA
1 ng/
µ
L
phosphors [22]
particle
fragment
D: Modified fluorescence (
∼
1
×
10
9
microscope
copies/
µ
L)*
Electrochemical
Electrochemical
-
T: 74 base DNA strand 50nM
(3
×
10
10
copies/
µ
L)*
sensor Motorola [23]
D: Electron transfer
Nanoparticles
Quantum dots [24]
-
T: Single-stranded DNA 10 nM
D: Fluor. microscope
(6
×
10
9
copies/
µ
L)*
6
×
10
6
copies
DNA-modified gold Single
T: Total genomic
probes with silver
particle
DNA/PCR products
(200 fM) (gen. DNA)
amplification
(0.0025)
D: Evanescent wave-
3000 copies
(Nanosphere) [25]
based scatter
(100 aM) (PCR)
measurements
8
×
10
6
copies
Resonant Light
Single
T: Spiked transcripts
Scattering
particle
D: CCD-based system
(170 fM)
(Genicon) [26, 27] (0.005)
Streptavidin-coated 5
6
×
10
7
copies
T: PCR products
gold nanoparticles
D: Laser illuminated
(
∼
1 pM)
with silver
scatter
amplification (AAT)
[28]
High resolution
T: Single-stranded DNA 3
.
2
×
10
6
copies
-
surface plasmon
D: SPR spectrometer
(54 fM)
resonance [29]
Gold nanoparticle-
0.5-20
T: Single-stranded DNA 10 pM
(6
×
10
6
copies/
µ
L)*
enhanced SPR [30]
D: Scanning angle SPR
SERS probes [31]
-
T: Single-stranded DNA 20 fM
D: Raman spectroscopy (1
.
2
×
10
4
copies/
µ
L)*
Gold nanoparticle-
-
T: Single-stranded DNA 500 fM
(3
×
10
5
copies/
µ
L)*
based electrical
D: Conductivity
detection [32]
*NOTE: copies/mL are reported for assays where reaction volumes were
unavailable.
