Chemistry Reference
In-Depth Information
Table 25.2
Continued
Protein
Experimental system
Altered glycosylation
Functional consequence
Fibronectin receptor
(
Enzymatic de -
N
- glycosylation
in human chronic erythroleu-
kemia K562 cells
Reduced extent of
N
- glyco-
sylation
Dissociation or altered as-
sociation of
α
5
β
1
- integrin)
1
- subunits,
inhibition of cell binding
to fi bronectin
α
5
β
Fibronectin receptor
(
Inhibition of
N
- glycan pro-
cessing (1 - deoxymannojirimy-
cin; swainsonine) in human
fi broblasts and breast carci-
noma cells from GnT-V
(Mgat5) null mice
Reduced extent of complex-
type
N
- glycosylation
Reduced cell and receptor
binding to fi bronectin;
reduced fi bronectin fi bril-
logenesis
α
5
β
1
- integrin)
Fibronectin receptor
(
Differential desialylation affect-
ing
Cleavage
of
α
2,8 - linked
Inhibition of integrin bind-
ing to fi bronectin
α
5
β
1
- integrin)
2,8 - linked oligosialic
acid chains in human mela-
noma G361 cells
α
sialic acid chains
Fibronectin receptor
(
Reconstitution of expression
of tumor suppressor p16
INK4a
in human pancreatic carci-
noma Capan - 1 cells
Altered galactosylation and
sialylation
Increased cell surface pre-
sentation and susceptibil-
ity to anoikis
α
5
β
1
- integrin)
Overexpression of GnT - V
(Mgat5) in transfected human
fi brosarcoma HT1080 cells
Increased level of
N
- linked
Inhibition of integrin clus-
tering, cell adhesion and
spreading, increased cell
migration
β
1
- Integrin in the
fi bronectin receptor
(
β
1,6 - branching
α
5
β
1
- integrin)
Glut - 2
GnT - IVa (Mgat4a) null mice
Reduced
N
- glycan branching
Reduced Glut - 2 half - life
at cell surface, insulin
resistance, type 2 diabe-
tes
FucT - VIII null mice
Reduced core fucosylation
of
N
- glycans
Growth retardation
in vivo
,
emphysema - like lungs,
MMP overexpression and
ECM downregulation
TGF -
β
R
TrkA/NGFR
Overexpression of GnT - III
(Mgat3) in transfected rat pheo-
chromocytoma PC12 cells
Increased levels of
N
- linked
Absence of NGF - induced
TrkA phosphorylation and
cell differentiation
β
1,6 - branching
The abbreviations used are: ECM, extracellular matrix; EGFR, epidermal growth factor receptor; ERK, extracellular
signal-regulated kinase; FAK, focal adhesion kinase; FucT-VIII,
1,6 - fucosyltransferase - VIII; GnT - III/IVa/V,
N
-
acetylglucosaminyltransferases; Glut -2, glucose transporter 2; MMP, matrix metalloproteinase; NGF, nerve growth
factor; NGFR, NGF receptor; TGF-
α
receptor; TrkA, tropomyosin-related kinase A.
For information on animal models, please see Chapter 23, especially Table 23.1 .
β
R, transforming growth factor-
β
