Chemistry Reference
In-Depth Information
Figure 5.1
Flow chart of
N
- glycan structural
analysis. Blue arrows show the strategy for
detailed structural analysis and yellow arrows
indicate the pathway for glycomic analysis.
N
-
Glycans are released from the glycoprotein
and derivatized to enhance the sensitivity of
chromatography or MS. Derivatized glycans are
isolated and their elution positions analyzed on
analysis. MS analysis provides information on
sugar components and allows precise differen-
tiation between glycan structures. The fragmen-
tation pattern obtained using MS/MS or MS
3
analysis often provides detailed structure anal-
ysis. For a glycomic analysis (yellow arrows) a
mixture of derivatized glycans is often pro-
cessed using MALDI-TOF/MS. This strategy is
