Agriculture Reference
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such as globulin and glutelin, and proteins associated with
seed maturation such as “early embryogenesis protein “and”
late embryogenesis abundant protein” and proteins related
to desiccation such as “abscisic acid-induced protein” and
“cold regulated protein”. The degradation of storage protein
happened mainly at the late stage of germination phase II
(48h), while that of seed maturation and desiccation associated
proteins occurred at the early stage of phase II (24h). In addition
to alpha-amylase, the unregulated proteins were mainly those
involved in glycolysis such as UDP glucose dehydrogenase,
fructokinase, phosphoglucomutase and pyruvate decarboxylase
(Yang et al. 2007).
Most of the high-yielding and high quality varieties cannot
be used in direct sowing because of low germination rate at
low temperature (LTG). The reduction in seedling growth of
rice due to low temperature is one of the major problems in
tropical and subtropical areas at high altitude as well as in areas
where cold mountain water is used for irrigation. In such areas,
water temperature during sowing is below 15ºC whereas the
optimum range for germination and early seedling growth of
rice is 25 to 35ºC. The delay in seeding emergence due to cold
water greatly increases seedling mortality and causes serious
decreases in yield and increases competition with weed. Studies
with low temperature germinability with a double haploid rice
(DH) population with 198 lines derived from anther culture of
F1 hybrid with indica line Zenshan 97B and a perennial japonica
AAV002863 was used. The germination rate in Zhenshan 97B
and AAV002863 was 79.7% and 30.1%, while DH population
ranged from 0 to 100% at 15ºC after 6 days (Fig. 7).
Proteases hydrolyze storage proteins to provide precursors
for perpetuating species. The protease activity of brown rice
increased sevenfold during 7 days of germination. It is highest
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