Biomedical Engineering Reference
In-Depth Information
- isopentenyl
mRNA
- methylthio
d
c
E-tRNA
Phe
A
37
A A G
P-tRNA
Phe
*
U
-3
U
-1
U
1
U
2
U
3
U
-2
e
P-tRNA
Phe
A790
*
A
37
U789
Mg(H
2
O)
2
6
U
+1
U
-1
mRNA
G926
Fig. 1.5
Stabilization of codon-anticodon interactions by cross-strand stacking of hypermodified
nucleotide 37 (ms
2
i
6
A37) in the A, P, and E sites. (
a
) 2F
o
-F
c
density contoured at 1.5s for ms
2
i
6
A37
of P-tRNA
GAA
Phe
. (
b
,
c
) Cross-strand stacking and tRNA-codon interactions in the A (
b
) and P (
c
)
sites in the elongation complex containing tRNA
GAA
Phe
. (
d
) Base pairing at the first position of the E
codon and stabilization by cross-strand stacking of the methylthio group of hypermodified nucleoside
ms
2
i
6
A37 of tRNA
GAA
Phe
in the elongation complex. (
e
) Anchoring of tRNA in the P site. A hydrated
magnesium ion is coordinated by the sulfur atom of ms
2
i
6
A37 of tRNA
GAA
Phe
in the P site, the mRNA
backbone phosphates of nucleotides at positions +1 and −1 and bases A790 and U789 of 16S rRNA
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