Comparisons of Techniques - Sample Preparation Topic for Transmission Electron Microscopy - page 232

Fig. 8.55 Collagen fibers (c)
after freeze fracture,
sublimation, and replication.
( J. Boumendil, CMEABG
UCB-Lyon 1 )
3.5.3 Comparison of “Chemical Fixation,” “Physical Fixation,”
and “Cryo-embedding” Techniques
Chemical Fixation (“Techniques” Chapter 2, Section 11), “Cryofixation” Physical
Fixation (“Techniques” Chapter 2, Section 12), and Cryo-embedding (“Techniq-
ues” Chapter 2, Section 10)
Bulk material: collagen fibers in a tissue (Figs. 8.56 and 8.57)
Fig. 8.56 Collagen fibers in a
tissue after chemical fixation,
embedding, ultramicrotomy,
and positive-staining contrast.
( J. Boumendil, CMEABG
UCBLyon I )