Biomedical Engineering Reference
In-Depth Information
Fig. 8.45
Ultrarapid
high-pressure cryofixation,
cryosubstitution, and
cryo-embedding in Lowicryl
K4M, ultramicrotomy and
positive contrast (uranyl in
aqueous solution + lead). The
cellular junction zone is
marked by the
broken lin
e.
(
J. Boumendil UCB-Lyon 1
)
a
b
Fig. 8.46
(
a
)and(
b
) Freeze fracture at 123 K using the double cupel technique. Platinum shad-
owing at 45
◦
and carbon replica. Cell junction between two enterocytes. (
A. Rivoire UCB-Lyon 1
)
intercellular space between the two membranes enabling it to be located. Lastly,
at the level of the gap junction, there are a large number of transmembrane pro-
teins (Fig.
8.46a)
which organize into connections in order to form communicating
canals from one cell to another. These canals ensure the rapid transport of chemical
or electrical elements between the two cells. These properties were described by
electrophysiologists before the corresponding structure had been seen.
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