Selection of Preparation Techniques Based on Material Problems and TEM Analyses - Sample Preparation Topic for Transmission Electron Microscopy - page 195

Table 7.9 Selection of the preparation technique based on the material and analysis type:
biological fine particles

Approach to Follow when Selecting the Preparation Type

Type of Material: Biological Fine Particles

Type of Analysis
Morphology, Labeling, Chemical Analysis

Immunolabeling

Select Fixation Method

Chemical Method Physical Method

Immunolabeling

Select Dehydration Method

Freeze-Drying,
Cryo-Substitution

Substitution

Select Embedding Method

Epoxy or Acrylic
at Room Temperature

Acrylic at
Low Temperature

Sectioning at
Room Temperature

Sectioning at
Low Temperature

Select Positive or
Negative Contrast

Select Shadowing Freeze Fracture

Observation Observation at Low Temperature

Room Temperature Low Temperature