Biomedical Engineering Reference
In-Depth Information
Fig. 6.52
Bright-field TEM
image of vitrified liposomes
in a frozen hydrated film,
showing a phase-contrast
inversion between the ice and
the liposome which is due to
the absence of osmium or
heavy metal (Ur or Pb)
commonly used for biological
preparation
(
G. Pehau-Arnaudet, Institut
Pasteur-CNRS, Paris
)
4.13 Artifacts Induced by the “Negative-Staining” Contrast
Technique
Fig. 6.53
Bright-field TEM
image of a liposome
suspension under negative
staining using uranyl acetate.
This image shows residues of
the dispersion agent (
white
spots
), which can be observed
around the liposome and are
sometimes superimposed over
the liposome structure. They
come from a component of
the suspension liquid that was
not eliminated (
J. Boumendil,
CMEABG - UCB Lyon 1
)
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