Biology Reference
In-Depth Information
4
Cell Entry of Enveloped Viruses
Fran¸ois-Loic Cosset*
,†,‡
and Dimitri Lavillette*
,†,‡
*Universit´ de Lyon, UCB-Lyon1, IFR128, Lyon, France
†
INSERM, U758, Lyon, France
‡
Ecole Normale Sup´rieure de Lyon, Lyon, France
I. Introduction
II. Architecture and Structure of the Viral Fusion Machinery
A. Membrane fusion according to the stalk-pore model
B. pH-dependent and -independent molecular switches
C. The entry process can be achieved by different
number of EnvGP
D. Structural classification: Class I, class II, and class III
fusion proteins
E. Common refolding process
F. Domain organizations/fusion domains
III. How Viruses Subvert Different Cell Proteins for Entry?
A. Definition of receptors, adsorption molecules, and cofactors
B. Use of multiple receptors—Receptor switch
C. Separation of the binding and fusion functions
IV. Basic and Translational Research Exploiting
Entry Properties of Viruses
A. Tropism properties and use of pseudoparticles in
gene therapy
B. Identification of viral cell entry receptors using
pseudoparticles
C. Determining the endocytosis pathway of entry and the
different cell proteins involved in entry by RNA interference
screens
