Brain Ischemic Injury in Rodents: The Protective Effect of EPO - Tissue-Protective Cytokines: Methods and Protocols - page 79

During surgical procedure, check regularly the degree of
anesthesia. The animal is well anesthetized when a loss of sensory/
reflex response is observed, i.e., nonresponse to tail pinching, or
paw pinching.

1. Stereomicroscope.
2. Homeothermic blanket system with rectal probe.
3. Laser Doppler monitor with 0.7 mm diameter probe (e.g.,
Laser Doppler Perfusion Monitor Moor Instruments) and a
probe holder.
4. Cranial headbolt (Kopf Instruments).
5. Bench Sterilizer.
6. Bench Hoover.
7. Blood gazes analyzer.
8. Computerized blood pressure monitoring (e.g., Blood Pressure
Display Unit, Stoelting).
9. Microcapnograph (e.g., Micro-CapnoGraph CI240, Columbus
Instruments).
10. Surgical Bone Drill System.
11. Electrosurgical unit (Harvard Apparatus).
12. Autoclave.
13. Animal shaver/hair clipper.
14. Common surgical tools set (fine biology tips forceps, hemo-
stats, retractor, alm retractor, mouth retractor, needle holder,
scalpel handle and blades, straight fine scissors, micro-scissors).
15. Cotton swabs.
16. Tape.
17. Syringes.
18. Needles.
19. Capillary tubes.
20. Nonabsorbable 5-0 silk black suture.
21. Nonabsorbable 3-0 silk black suture.
22. Carbon still burrs.
23. Polyethylene catheters.

2.3 Equipment

1. Isoflurane.
2. 30% oxygen/70% nitrous oxide.
3. Saline (0.9% sodium chloride).
4. Surgical betadine.
5. Heparin.

2.4 Reagents