Biology Reference
In-Depth Information
3
Methods
The flow chart describing the experimental procedures is shown in
Fig.
1
.
3.1
Infection
1. During the injection procedure, keep
P. berghei
ANKA inocu-
lum at 37°C using a mobile water bath.
2. Take a 1 mL sterile syringe with 0.33 × 12 mm needle.
3. Fill in the syringe with 200
L
P. berghei
ANKA suspension.
4. Hold the mice by the neck using forefinger and the skin using
other fingers.
5. Bulge the abdomen of the mice.
6. Proceed to IP injections of 200
μ
μ
L
P. berghei
ANKA suspen-
sion per mouse.
1. At day 6, randomized mice in four groups of 15 mice.
2. At day 6, day 7, and day 8, for a mouse weighing 20 g, proceed
to intraperitoneal injection of:
200
3.2
Treatment
μ
L of saline to control group.
●
200
μ
L of Epo 100 IU/mL per day to Epo group.
●
100
μ
L of artesunate 8.0 g/L per day to AS group.
●
200
μ
L of Epo 100 IU/mL and 100
μ
L of artesunate
●
8.0 g/L per day to AS-Epo group.
Fig. 1
Flow chart of murine cerebral malaria infection and treatment with Epo
and artesunate
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