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22. The fraction of O
2
in the plethysmography chamber should be
gradually decreased from 21 to 6% O
2
during 15 min in order
to avoid mice death due to a too rapid O
2
deprivation. However,
if mice are exposed to 10% O
2
or higher during the hypoxia
regimen, the decrease in the fraction of O
2
do not necessitate
intermediate step(s) and could be done directly from 21 to
10% O
2
without risk of asphyxia for mice.
23. It is highly suggested to not turn off the O
2
and CO
2
analyzers
and the amplifier after each utilization in order to maintain
their functioning temperature as stable as possible.
24. Proceed as recommended by the supplier and, importantly, do
not use any chemicals that may damage the plethysmograph
chamber's integrity (such as ethanol) or influence respiration
(or stress) of the next experimental mice.
25. There is several ways to analyze plethysmography data, depend-
ing on the experimental context or the plethysmography set-
up used. We present and propose here one of these possibilities,
which is appropriate and relevant for the whole-body unre-
strained plethysmography carried out on adult mice with the
set-up we presented. However, this is not the only appropriate
method to analyze this type of results.
Acknowledgments
The authors would like to thank Drs Vincent Joseph and Cécile
Julien as well as Mr Raphaël Lavoie and Miss Hanan Khemiri for
helpful discussions and valuable advices. J.S. is supported by the
Respiratory Health Network of the FRSQ (Fonds de la Recherche
en Santé du Québec), the Foundation of Stars for the Children's
health research, the Molly Towell Perinatal Research Foundation
(MTPRF). M.G. is supported by the Zurich Center for Integrative
Human Physiology (ZIHP).
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