Biology Reference
In-Depth Information
a
0.05
0.00
21% O
2
−0.05
0.05
0.00
6% O
2
−0.05
10 sec
b
270
1.2
260
240
250
1.1
*
*
220
*
1.0
230
200
*
210
0.9
180
160
190
0.8
140
120
100
0.7
170
150
0.6
Vehicle
Epo
Vehicle
Epo
Vehicle
Epo
Vehicle
Epo
Vehicle
Epo
Vehicle
Epo
21% O
2
6% O
2
21% O
2
6% O
2
21% O
2
6% O
2
c
14
14
12
12
10
10
8
8
6
6
4
4
2
2
0
0
Vehicle
Epo
Vehicle
Epo
Vehicle
Epo
Vehicle
Epo
21% O
2
6% O
2
21% O
2
6% O
2
Fig. 2
Typical results obtained by analysis of data from whole-body unrestrained plethysmography achieved
on female adult mice. (
a
) Representative examples of respiratory traces registered at 21% O
2
(normoxia) and
at 6% O
2
(severe hypoxia). Example of results obtained for ventilatory (
b
) and metabolic (
c
) parameters in mice
injected with Epo or vehicle (i.p.). Significant difference (
p
< 0.05) between vehicle and Epo groups are delin-
eated by an
asterisk
smaller animal by reducing the total air volume in the animal
chamber. Moreover, controlling the temperature within the
plethysmography chamber is necessary when experiments were
carried out on younger animals that do not have yet the physi-
ological capacity to thermoregulate adequately (<15 postnatal
days in mice and rats).
2. Plethysmography can be achieved in a whole-body or double-
chamber/head-out set-up. The whole-body system allows to
make measurements on unrestrained and conscious animal
during a long period of time without anesthetic bias. The rela-
tively reasonable size of mice (and rats!) allows the use of this
approach.
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