Using Plethysmography to Determine Erythropoietin’s Impact on Neural Control of Ventilation - Tissue-Protective Cytokines: Methods and Protocols - page 295

2 Materials

2.1 Plethysmogra-
phy System
Components

An overview of the plethysmography system as well as the connec-
tions between its components is schematized in Fig. 1 .

1. Pump for regulated air supply (EMKA Technologies)
(see Note 3).
2. Flowmeter (E & E Process Instrumentation) (see Note 4).
3. Plethysmography chamber for whole-body plethysmography
on unrestrained mice (EMKA Technologies).
4. Thermocouple monitoring thermometer with probe
(see Note 5).
5. Differential pressure transducer (EMKA Technologies)
(see Note 6).
6. Amplifier for differential pressure transducer's signal (EMKA
Technologies).
7. Humidity meter (see Note 7).
8. Subsampling pump (Sable Systems International) (see Note 8).
9. CO 2 analyzer (Sable Systems International).
10. O 2 analyzer (Sable Systems International).

Fig. 1 Schematic overview of the system used for whole-body unrestrained plethysmography in adult mice.
The tubings containing arrows represent the air path, while dark lines symbolize the electronic connections
transporting analog data which are converted by the acquisition system into digital data to provide the experi-
mental parameters included in the ovals