Myocardial Infarction: Cardioprotection by Erythropoietin - Tissue-Protective Cytokines: Methods and Protocols - page 263

Table 2
Animal experiments with in vivo models of ischemia-reperfusion

Time of treatment End-points

First author
and references

EPO, type and
doses (IU/kg)

Duration of
occlusion (min) Date Species Relative to Isch/Rep End-points Outcomes

Calvillo ( 18 ) 2003 Rat rhEPO, 5,000 i.p.

multiple doses

24 h, 0.5 h before
Isch, then daily

30 At 7 days:

LVEDP ↓ p < 0.001

Number of CM ↑ p < 0.01

Parsa ( 19 ) 2004 Rabbit EPO, 1,000

or 5,000 single
dose i.v.

12 h before Isch, at
Isch or start Rep

45 At 45 min:

MI size ↓ p < 0.005 ( all

EPO groups )

LV function ↑ p < 0.05 ( only

EPO 12 h before
or at Isch )

CM apoptosis ↓ p < 0.05 ( only

EPO 12 h before
Isch )

Abdelrahman
( 20 )

2004 Rat EPO 300 i.v. Start of Rep or of

resuscitation

20 At 2 h:

MI size ↓ p < 0.05

Renal and liver
function

↑ p < 0.05

Lipsic ( 21 ) 2004 Rat EPO 5,000 i.p. 2 h before Isch or at

start Isch or right
after Rep

45 At 24 h:

MI size ↓ p < 0.05

LV function p < 0.05 (=NS for

2 h before Isch)

CM apoptosis ↓ p < 0.05(=NS for

2 h before Isch)

Rui ( 22 ) 2005 Mouse rhEPO 5,000 i.p. 24 h and 30 min

before Isch

30 At 4 h: myocardial

MPO

↓ p < 0.05

(continued)