Photoreceptor Degeneration in Mice: Adeno-Associated Viral Vector-Mediated Delivery of Erythropoietin - Tissue-Protective Cytokines: Methods and Protocols - page 232

2.3.2 Subretinal
Injections

1. Hamilton syringe 95RN 5 L (Hamilton Company, USA).

2. Dumont tweezers #5, 11 cm long, 0.05 × 0.01 mm bent (45°)
tips.
3. Vannas scissors, 82 mm long, 9.5 mm sharp straight tips.
4. 30 gauge needles (to be mounted on 1 mL syringes).
5. Removable 33 gauge needle to be mounted on the Hamilton
syringe (Hamilton Company, USA).
6. Dissecting microscope.
7. Fiber optic light sources.
8. Ophthalmic antibiotic ointment.

μ

1. Hamilton syringe 710RN 100 μ L (Hamilton Company,

2.3.3 Intramuscular
Injections

USA).
2. Sterile stiff support.
3. Removable 30 gauge needles to be mounted on the 710RN
Hamilton syringe (Hamilton Company, USA).
4. Dumont tweezers #5, 11.5 cm long, 0.17 × 0.10 mm serrated
tips.
5. Mini dissecting scissors 9.5 cm long, with sharp fine tips.
6. Sterile razor blades.
7. A topical antibiotic ointment.
8. Catgut (monofilament polyglyconate synthetic absorbable
suture).
9. Adhesive gauze.

1. Lewis rats (see Note 7).
2. A custom-made apparatus for light damage composed of: eight
36-W white bulbs, two transparent Plexiglas sheets, four trans-
parent Plexiglas cages, a wired support to lodge the cages (see
Note 8).
3. Luxometer (see Notes 9 and 10).

2.4 Light Damage

1. A room to work under red dim light.
2. A completely obscured and highly ventilated box to adapt the
rats to the dark.
3. A stereotaxic support.
4. Avertin (see Subheading 2.3.1 ).
5. A heating pad.
6. Two reference needle electrodes.
7. One ground needle electrode.
8. Two gold-plated electrodes.

2.5 Electroretinog-
ram Recordings