Biology Reference
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Fig. 1
EPO stimulates angiogenesis in the chick embryo CAM. CAM of 12-day-old
chick embryo incubated for 4 days with a gelatin sponge adsorbed with 10 U of
rHuEpo (
a
) compared to vehicle (
b
). Note the presence of an increased number of
blood vessels with a radially arranged spoked wheel pattern around the implant
Ten sections are analyzed for each CAM specimen by observ-
ing every third section within 30 serial slides. For each section,
six randomly chosen microscopic fields are evaluated for
the number of intersection points occupied by microvessels.
Then, mean values ±1 standard deviation are determined for
each CAM specimen. The microvessel density is expressed as
percentage of intersection points occupied by microvessels.
Statistically significant differences between the mean values of
the intersection points in the experimental CAMs and control
ones are determined by Student's
t
-test for unpaired data.
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