ARA290 in a Rat Model of In fl ammatory Pain - Tissue-Protective Cytokines: Methods and Protocols - page 212

Fig. 1 Illustration of the neuritis induction. ( a ) A strip of Gelfoam saturated in
complete Freund's adjuvant is carefully fed under the cleared length of the sciatic
nerve in the mid thigh (proximal to the trifurcation of the nerve). ( b ) The Gelfoam
is drawn around the nerve and ( c ) the ends are loosely wrapped over the top. The
photo insert shows the Gelfoam surrounding the sciatic nerve
27. Close the overlying fascia with 4-0 coated Vicryl sutures. Two
simple interrupted sutures (surgical knots) are usually required.
Use the Dumont angled forceps to support the fascia.
28. Close skin with 4-0 coated Vicryl sutures. Four interrupted
sutures are usually required. Use the needle holders and the
curved forceps to support the skin (see Note 8).
29. Spray surgical site with Betadine powder.