Deciphering the Intracellular Signaling of Erythropoietin in Neuronal Cells - Tissue-Protective Cytokines: Methods and Protocols - page 180

polished glass pipettes (check under microscope for sharp
edges and opening of >300 m), too many times, too forceful

to create bubbles will significantly decrease the number of neu-
rons. Moreover, remaining neurons might not be viable and
become necrotic within few days in culture.

μ

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