Biology Reference
In-Depth Information
4. The tail can be warmed up with hot water or leaving the cage
under infrared lamp for about 5 min to ease the detection of
the lateral tail vein. Mice are held in the restrainer for i.v.
injection.
5. During the course of the disease mice are not able to move
properly. The food pellet and the water need to be placed in a
petri dish into the cage to enable sick mice to eat and drink.
6. It is almost impossible to put a healthy mouse on its back,
because it will turn over immediately to regain the upright
position. When a mouse is sick, it will lie on its back at least for
a few seconds, or it will be unable to regain the upright posi-
tion. Any impairment of the righting reflex is scored as 2.
Acknowledgments
This work was realized as part of the TC2N “Trans Channel
Neuroscience Network” Interreg IV A 2 Mers Seas Zeeëns pro-
gram, “Investing in your future” crossborder cooperation pro-
gramme 2007-2013 part financed by the European Union
(European Regional Development Fund).
References
1. Trapp BD, Nave KA (2008) Multiple sclerosis:
an immune or neurodegenerative disorder?
Annu Rev Neurosci 31:247-269
2. Makar TK, Bever CT, Singh IS et al (2009)
Brain-derived neurotrophic factor gene deliv-
ery in an animal model of multiple sclerosis
using bone marrow stem cells as a vehicle. J
Neuroimmunol 210:40-51
3. Brines ML, Ghezzi P, Keenan S et al (2000)
Erythropoietin crosses the blood-brain barrier
to protect against experimental brain injury.
Proc Natl Acad Sci U S A 97:10526-10531
4. Bartels C, Spate K, Krampe H, Ehrenreich H
(2008) Recombinant human erythropoietin:
novel strategies for neuroprotective/neuro-
regenerative treatment of multiple sclerosis.
Ther Adv Neurol Disord 1:193-206
5. Sargin D, Friedrichs H, El-Kordi A et al (2010)
Erythropoietin as neuroprotective and neu-
roregenerative treatment strategy: comprehen-
sive overview of 12 years of preclinical and
clinical research. Best Pract Res Clin
Anaesthesiol 24:573-594
6. Ehrenreich H, Fischer B, Norra C et al (2007)
Exploring recombinant human erythropoietin
in chronic progressive multiple sclerosis. Brain
130:2577-2588
7. Agnello D, Bigini P, Villa P et al (2002)
Erythropoietin exerts an anti-inflammatory
effect on the CNS in a model of experimental
autoimmune encephalomyelitis. Brain Res
952:128-134
8. Sattler MB, Merkler D, Maier K et al (2004)
Neuroprotective effects and intracellular sig-
naling pathways of erythropoietin in a rat
model of multiple sclerosis. Cell Death Differ
11(Suppl 2):S181-S192
9. Diem R, Sattler MB, Merkler D et al (2005)
Combined therapy with methylprednisolone
and erythropoietin in a model of multiple scle-
rosis. Brain 128:375-385
10. Li W, Maeda Y, Yuan RR et al (2004) Beneficial
effect of erythropoietin on experimental
allergic encephalomyelitis. Ann Neurol 56:
767-777
11. Gold R, Linington C, Lassmann H (2006)
Understanding pathogenesis and therapy of
multiple sclerosis via animal models: 70 years
of merits and culprits in experimental autoim-
mune encephalomyelitis research. Brain
129:1953-1971
12. Steinman L, Zamvil SS (2006) How to suc-
cessfully apply animal studies in experimental
allergic encephalomyelitis to research on mul-
tiple sclerosis. Ann Neurol 60:12-21
13. Furlan R, Cuomo C, Martino G (2009) Animal
models of multiple sclerosis. Methods Mol
Biol 549:157-173
Search WWH ::




Custom Search