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Fig. 2
Endotracheal tubes. (
a
) ET tube for rats is prepared using 14 G/16 G cath-
eter and No. 2.5 ET tube. In rats the elastic tape that is tied around the proximal
part of the catheter is secured to the lower jaw after intubation. (
b
) ET tube for
mice prepared from 21 G catheter and PE tubes. Note the 5-0 silk suture knot
that lodges into the superficial groove on the proximal PE tube (that is later con-
nected to the black “Y” connector of the ventilator tubings). After intubation the
ET tube is sutured to the lower jaw by taking a stitch through the skin
2. Mice: As it is difficult to visualize the vocal cords in mice we
use the technique described by Michael et al. (
49
) for intuba-
tion (
50
). The mouse is induced in the anesthesia chamber
with 3% isoflurane in 100% oxygen for 3-5 min. It is then
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