Biology Reference
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Fig. 1
Immobility in the forced-swim test (FST) is decreased by 4 days of EPO administration in rats. EPO
(500 U/kg, i.p.) or vehicle (0.1% BSA in PBS) were administered to rats on 4 consecutive days, with the last
dose 4 h prior to the day 2 swim test. Data are presented for two 5-min intervals of the test. *
p
< 0.05,
Student's
t
test,
n
= 6
EPO (500 U/kg) is injected intraperitoneally (i.p.) once per day
for 3 consecutive days. Vehicle is similarly administered to a second
group of mice (8/group) (see Notes 3 and 4).
3.2
Mouse FST
Swim Test
1. EPO and vehicle are administered as on the prior 3 days to the
same groups of mice. This is the fourth consecutive day of
treatment. Time injections so that pairs of mice are injected
every 15 min. Swim testing starts for the first pair of mice 4 h
after they are injected.
2. The two beakers are filled with the room temperature water to
the 10 cm mark and beakers are placed with a white divider
between them, on a cart or table at one end of the room with
a plain white surface (such as a bare wall) behind them. Visual
stimuli should be the same on the outer sides of both beakers.
The camera is set on a tripod at the other end of the room
where the experimenter also sits (equidistant from both bea-
kers). Ensure that normal overhead lighting falls evenly on
both beakers.
3. All mice to be tested are brought in their homecages into a
quiet holding room near the testing room. Mice acclimate in
the holding room for 1 h.
4. Two mice at a time are brought from the holding room into
the testing room and lowered by the tail into the two beakers
(see Note 5). The experiment timer is started.
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