Biology Reference
In-Depth Information
of a strain can influence the final infarct size, it could also
impact the dynamic of evolution of the lesion at the acute
stage. Bardutsky and colleagues showed, using MRI, that the
spatiotemporal evolution of the ischemic penumbra is differ-
ent between two strains of rats of the same age and without
other comorbidity (
39
).
3. Several concomitant pathologies are known to exacerbate the
ischemic brain lesion. The most often encountered comor-
bidities are for example arterial hypertension, diabetes and
cardiovascular diseases. Hypertensive animals have been
shown to display more extended lesions and earlier disappear-
ance of the ischemic penumbra compared to normotensive
ones (
40, 41
).
4. Almost all the experimental studies involve the exclusive use of
males. The use of females adds technical complications. As
female's hormones could influence per se the evolution of the
ischemic lesion, the menstrual cycle of the females rats need to
be monitored and the experiments performed at a specific
point of the estrus cycle (
42, 43
).
5. How to prepare a thermofusible glue-coated filament of
0.380 mm diameter and 2.5 mm length
Materials
(a) Nylon monofilament (0.18 mm in diameter)
(b) Thermofusible glue (melting temperature: 70°C)
(c) Microscope
(d) Homemade calibrated hole
(e) Soldering iron
(f) Forceps
(g) Digital caliper (World Precision Instrument)
Procedure
(a) Cut the nylon monofilament into 3 or 4 cm segments
(b) Under the operating microscope, coat the extremity of
the nylon thread with the thermofusible glue (melting
temperature: 70°C) by passing it through a calibrated
hole made in a heated piece of metal fixed on the solder-
ing iron.
(c) Special attention is made to obtain a perfect cylinder with-
out asperity.
(d) The extremity of the filament is examined under the micro-
scope after cooling and drying. All the shapes that are not
perfectly smooth and constant are discarded.
(e) The diameter of the tip is verified using a digital caliper.
Search WWH ::
Custom Search