Agriculture Reference
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Fig. 26.3.
Cells of the petiole of leaf 1 of 3-week-old tomato seedlings.
a
,
c
,
e
Cells labelled
with the fluorescent dye LY via the glass micropipette used for intracellular recording:
a
an
in situ epidermal cell;
c
an in situ cortical cell;
e
an in situ sieve-tube element: most of the
dye has moved in the direction of the main stem, the
asterisk
indicates a sieve plate.
Scale
bars
represent 100 µm.
b
,
d
,
f
Electrical recordings from the same cells as shown in
a
,
c
and
e
;
b
epidermal cell;
d
cortical cell;
f
sieve-tube element.
S
surface electrode (cotton thread
soaked in 10 mM KCl connected to an Ag/AgCl electrode),
B
bath electrode (saline bath
connected to an Ag/AgCl electrode by an agar/3 M KCl salt bridge),
I
intracellular electrode
(glass micropipette back-filled with a 1% aqueous solution of LY followed by 3 M LiCl and
an Ag/AgCl electrode). Heat was applied to cotyledon 1 for about 30 s causing wounding
and a small artefact to appear on the traces (Reproduced from Rhodes et al. 1996, with the
permission of Springer-Verlag)
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