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Fig. 25.6.
Pattern of AK isozymes from
C. rubrum
(ecotype 374) after chromatography on
(diethylamino)ethyl (
DEAE
) cellulose.
To p
Seedlings grown on Hoagland's solution with
0.5% agar at 6,500 lx continuous white fluorescent light for 24 days.
Bottom
The same
conditions with one inductive dark period of 12 h, 5.5 days after sowing. The abundance
of the different isozymes in the cellular compartments changes after flower induction;
in particular the relation between chloroplasts (AK V) and mitochondria (AK I). Isozyme
AK IV (nucleus) was not detected (Wagner et al. 1983).
AK I
mitochondria,
AK II
cytoplasm,
AK III
chloroplasts,
AK IV
nucleus,
AK V
chloroplasts,
P
phosphatase
used for the control of development, i.e. via electrogenic induction of flow-
ering by DC pulses (Wagner et al. 2004).
At the apex, photoperiodic conditions inducing flowering have been
studied at the cellular level in
C. rubrum
(Sect. 25.3). There are very early
changes in calcium and pH patterning (Albrechtová et al. 2003) and carbo-
hydrate metabolism, which could lead to an increase in osmotic pressure
inthecellsoftheapicalmeristem,andthusbuildadrivingforceforwater
transport (Albrechtová and Wagner 2004). It is well known that aquaporins
are involved in regulating water relations, and studies in
C. rubrum
revealed
the expression of a novel aquaporin (CrAQP) with a transient increase in
the expression a few hours before the maximal increase in the size of the
meristem (Albrechtová and Wagner 2004; Albrechtová et al. 2004). It was
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