Cell Surface Engineering Using a Layer-by-Layer Nanofilm for Biomedical Applications - Cell Surface Engineering: Fabrication of Functional Nanoshells - page 232

shear stress during the inkjet-printing process. 121 Accordingly, we have to
carefully consider cell viability and function during tissue engineering.
As we described before, we recently discovered a simple and unique
bottom-up approaches ''hierarchical cell manipulation'' and ''cell accumu-
lation technique'' to develop 3D-tissue constructs by the fabrication of nano-
ECM films onto the cell surfaces. Since the coating of LbL-assembled

d n 8 y 4 n g | 6

.

Figure 11.9

Schematic illustration of the effect of physical stress on (a) uncoated
cells or (b) cells with films by LbL assembly (A). Cell viability of HepG2
with or without FN-G films for 9 step LbL assembly (B). Cell viability of
HepG2 with ( J ) or without (n) FN-G films at each centrifugation was
plotted (n ¼ 3). Cell viability was measured by trypan blue staining
and was calculated by the ratio of the number of living cells to the
total number of cells. Error bars denote standard deviations.
Reproduced with permission from Ref. 122.