Biomedical Engineering Reference
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Figure 11.7
Schematic illustration of HUVEC tube formation by sandwich culture
with NHDF tissues (A). Immunological staining image with antifactor
VIII antibody for 4L-NHDF-1L-HUVEC-4L-NHDF tissues after 7 days
of incubations (B). CLSM image of HUVEC and LEC networks im-
munologically staining with anti-CD31 and anti-LYVE-1 antibodies,
respectively (C).
.
image analysis. This would provide a benefit for the surrounding cells, be-
cause cells in living tissues require endothelial tube networks within a
perimeter of 100-150 mm to supply oxygen. Moreover, lymph capillary net-
work structures could also be obtained by employing lymph endothelial cells
(LECs) instead of HUVECs. When we use both blood and LECs for the vas-
cularization process, individual networks in 3D-NHDF tissue constructs were
successfully obtained. The obtained vascularized constructs were stable
during long-term culture for at least 3 weeks. These microvascular and
-lymph networks in multiwell plates will be a powerful tool for pharma-
ceutical and pathophysiological applications.
11.5.7 Inkjet-Printing Approaches
The cell-based approaches allow us to build a 3D-tissue construct with high
cell density that shows cell-cell interactions. However, the obtained con-
structs are still far from the real in vivo tissues. In particular, the mechanical
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