Magnetic Decoration and Labeling of Prokaryotic and Eukaryotic Cells - Cell Surface Engineering: Fabrication of Functional Nanoshells - page 202

The entire procedure can be performed in less than 30 min, and both cell
fractions - magnetically labeled and untouched cells - are ready for further
use, such as flow cytometry, molecular biology, cell culture, transfer into
animals, or clinical cellular therapy. 56,82
The large-scale magnetic separation of target cells using magnetic nano-
particles can be performed in the automated CliniMACS device (Miltenyi
Biotec) that enables magnetic cell selection in a closed and sterile system
(Figure 10.9). The use of clinical-grade isolation or depletion of cells is now a
standard technique established in many cellular therapy centers.
An alternative procedure is based on the use of magnetically responsive
microparticles, such as Dynabeads, bearing immobilized primary or sec-
ondary antibodies or (strept)avidin. On the small scale, labeled cells
(Figure 10.10) can be easily separated using standard magnetic separators.
The Isolex@ 300 System (Baxter, USA) is a semiautomated magnetic cell
separation system designed to select and isolate CD34 positive cells, ex vivo,
from mobilized peripheral blood using anti-CD34 monoclonal antibody and
superparamagnetic Dynabeads microspheres. 56
The positively selected cells may, in many cases, not show any interference
from the larger magnetic particles and may also be analyzed or used with the

d n 8 y 4 n g | 5

.

Figure 10.9 The automated system for clinical isolation of human cell subsets
(CliniMACS, Miltenyi Biotec, Germany).
Reproduced, with permission, from Ref. 113.