Biology Reference
In-Depth Information
D3S1358
vWA
D16S539
D2S1338
XY
D8S1179
D21S11
D18S51
D19S433
THO1
FGA
The allelic ladder of the AmpF
STR
SGM Plus
kit contains all the common alleles
Figure 6.9
Figure 6.10
The comparison of an unknown allele with the allelic ladder allows the THO1 alleles
to be classified as 7 and 9.3. The size of the unknown allele and the allele in the allelic ladder are
not identical but fall within 0.5 bp of each other. The 0.5 bp match windows are indicated by the
shaded areas
and require further analysis. This comparison of unknown peaks to the allelic ladder
can be done manually or by using the Genotyper
(Figure 6.10) or GeneMapper
TM
ID
software (Applied Biosystems), which will compare all the unknown alleles in
the profile to the allelic ladder.
The final result is a profile where alleles have been assigned to all of the peaks in
the profile (Figure 6.11).
The STR profiles should generate the same allele designations regardless of the lab-
oratory where the analysis took place or the variations in the methodology that may