Biology Reference
In-Depth Information
(a)
(b)
(c)
Figure 7.14 The same DNA extract was amplified in the presence of (a) 0 ng/ µ l humic acid;
(b) 8 ng/ µ l humic acid; and (c) 12 ng/ µ l humic acid. The humic acid causes inhibition; at a
concentration of 8 ng/ µ l the profile has a similar appearance to the degraded profile in Figure 7.10,
with reduced peak heights at the larger loci; at a concentration of 12 ng/ µ l the profile is almost
completely absent
References
1. Shinde, D. Lai YL, Sun FZ, Arnheim N. (2003) Taq DNA polymerase slippage mutation rates
measured by PCR and quasi-likelihood analysis: (CA/GT)(n) and (A/T)(n) microsatellites.
Nucleic Acids Research , 31 , 974 -980.
2. Hauge, X.Y. and Litt, M. (1993) A Study of the origin of shadow bands seen when typing
dinucleotide repeat polymorphisms by the PCR. Human Molecular Genetics , 2 , 411 -415.
3. Schlotterer, C. and Tautz, D. (1992) Slippage synthesis of simple sequence DNA. Nucleic
Acids Research , 20 , 211 - 215.
4. Applied Biosystems (1999) AmpFISTR SGM Plus PCR Amplification Kit - User's Manual.
5. Gill, P., Sparkes, R. and Kimpton, C. (1997) Development of guidelines to designate alleles
using an STR multiplex system. Forensic Science International , 89 , 185 - 197.
Search WWH ::




Custom Search