Biology Reference
In-Depth Information
(a)
(b)
Figure 7.13 The D18S51 locus dropped out when profiled using (a) the AmpF STR Identifiler ,
but was successfully analysed using (b) the AmpF STR MiniFiler
very low PCR amplification is carried out in duplicate or even triplicate to minimize
the possibility of generating an incorrect profile.
To assist with the analysis of degraded DNA, a series of multiplexes have been
developed with the primers positioned close to the core repeats of the STRs, thereby
minimizing the lengths of the amplicons [31 - 35]. The combination of the stan-
dard STR multiplexes with the shortened amplicons can increase the amount of
information recovered from a particular sample (Figure 7.13).
PCR inhibition
In addition to DNA degradation, if the DNA extraction procedure does not remove
chemicals that interfere with the PCR, inhibition can result (Figure 7.14) (see
Chapter 5). Inhibition can be difficult to differentiate from DNA degradation, although
the use of real-time quantification can be used to detect inhibitors (see Chapter 4).
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