Methods - Free-Radical-Induced DNA Damage and Its Repair - page 469

Methods

13.1 General Remarks 485

13.2 Detection of DNA Damage 485

13.2.1 Excision of Damaged Bases by Acid Hydrolysis 485

13.2.2 Excision by Enzymatic Hydrolysis 486

13.2.3 Detection of DNA Lesions

by Gas Chromatography/Mass Spectrometry

487

13.2.4 Determination of DNA Lesions

by Liquid Chromatography 488

13.2.5 MALDI-TOF 488
13.2.6

Capillary and Pulsed Field Gel Electrophoresis 488

13.2.7 32 P-Postlabeling 489
13.2.8 Detection of Hydroperoxides 490
13.2.9 Malonaldehyde, Base Propenals
and Related Compounds 491
13.2.10 Guanidine Derivatives 491
13.2.11 Immuno Assays 492
13.2.12 Atomic Force Microscopy 492

13.2.13 Comet Assay 492
13.2.14 Detection of SSBs and DSBs
with the Help of Supercoiled Plasmids 493

13.2.15 Detection of Clustered Lesions 493

13.3 Pulse Radiolysis and Laser Flash Photolysis 493

13.4 Gas Explosion and Rapid-Mixing Techniques 497

References 498