Tissue Engineering: Focus on the Musculoskeletal System - Biomaterials Science: An Integrated Clinical and Engineering Approach - page 199

1)

A

NCO

POSS-(PLA 20 ) 8
1 eq.

+ HO N 3 +

OCN

2 eq. 5 eq.

HN GRGDSK(FITC)

N 3

O

2)

N 3

POSS-SMP-20-Az POSS-SMP-20-Peptide

B

10000 3

POSS-SMP-20

2.5

1000

POSS-SMP-20-Az
POSS-SMP-20-Peptide

2

100

1.5

10

1

1

0.5

0

0.1

30 40 50 60 70 80 90 100

Te mperature (°C)

C

POSS-SMP-20-Az Control POSS-SMP-20-Peptide (FITC-labeled)

DIC FI. DIC FI.

400 µm 400 µm 400 µm 400 µm

FIGuRE 9.2 (See color insert.)
Chemical modification of POSS‐SMP with a bioactive peptide: (A) Synthetic scheme illustrat-
ing the introduction of azido groups during the covalent cross‐linking of POSS‐(PLA20)8
and subsequent conjugation of fluorescently labeled integrin‐binding peptide to POSS‐SMP
via “click” chemistry. (B) Storage modulus (E′)‐temperature curves and loss angle (Tan δ)‐
temperature curves (denoted by black arrows) of POSS‐SMP‐20, POSS‐SMP‐20‐Az, and POSS‐
SMP‐20‐Peptide. (C) Differential interference contrast (DIC) and fluorescent (Fl) micrographs
confirming the covalent conjugation of the fluorescently labeled peptide via click chemistry.
(From PNAS 107:7652-7657, 2010. With permission from the Publisher.)