Biomedical Engineering Reference
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(A)
(B)
50 µm
20 µm
FIGuRE 8.1 (See color insert.)
In-vitro construction of engineered vascularized cardiac muscle using a multi-cellular strategy of hES-CMs, endothelial cells, and embryonic fibro-
blasts seeded within a porous polymer scaffold of PLLA/PLGA. (A) The endothelial cells (vWF, green) within the scaffold self-organized to lumen
vessel structures located in close proximity to the hES-CMs (troponin I red). (B) Higher magnification reveals that the hES-CMs matured to a certain
degree, presenting developed cytoplasm and sarcomeric pattering (troponin I, red). Nuclei are stained with DAPI (blue).
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