Recovery and Extraction of Polyhydroxyalkanoates (PHAs) - Polyhydroxyalkanoate (PHA) Based Blends, Composites and Nanocomposites - page 61

PHA
(%)

Recovery
yield (%)

recovery
(Da)

recovery
(Da) Microorganism Recovery treatment Purity (%) Reference

Cupriavidus necator 58.8 Non-halogenated solvent extraction using isoamyl

propionate, propyl butyrate, isoamyl valerate or
isoamyl isoamylate (isovalerate) (3-methyl-1-
butanoate of 3-methyl-1-butanol)

— 90.0 1 000 000 550 000-

735 000

57

Cupriavidus necator 60-75 Non-halogenated solvent extraction using isoamyl

alcohol

— 98.0 1 000 000 650 000-

780 000

58

ND b

Cupriavidus necator — Supercritical carbon dioxide (1 time), 200 atm,

40 1C, 100 min and 0.2 mL methanol

— 89.0 ND 31

Cupriavidus necator — Supercritical carbon dioxide (2 times) with 1% (v/v)

toluene as a modifier, 200 atm, 30 1C

— 81.0 ND ND 30

Recombinant
Cupriavidus necator

— Non-halogenated solvent extraction using toluene,

1h,901C

98.0 90.0 1 000 000 390 000 59

Cupriavidus necator — Bromelain; pancreatin 89.0 90.0 ND ND 4

Burkholderia sp. PTU9 — Papain 89.0 78.0 ND ND 22

Bacillus flexus — Sonication 92.0 20.0 ND ND 60

Bacillus flexus — Cell fragility by alkaline hydrolysis — 50.0 ND ND 61

Azotobacter vinelandii
UWD

— Cell fragility by alkaline hydrolysis — 94.0 ND ND 38

Recombinant
Escherichia coli

77 0.2 M NaOH, 1 h, 30 1C 98.5 92.0 2 200 000 1 900 000 21

Cupriavidus necator 32 0.1 M NaOH, 30 1C, 1 h 46.0 80.0 ND ND 62

Recombinant
Pseudomonas putida
GPp104

— 0.5 M NaOH, 30 1C, 1 h 75.3 67.2 ND ND 11

Pseudomonas putida 10 0.1 M NaOH, 2 h, 22 1C 44.0 92.0 82 000 82 000 63

Cupriavidus necator
H16

70 1 M NaOH, 3 h, 60 1C 78.0 45.0 ND ND 64

Recombinant

Cupriavidus necator E 30

0.05 M NaOH, 3 h, 0 rpm, 4 1C 96.6 96.9 169 000 147 000 53

Recombinant

Cupriavidus necator E 30

Water, 1 h, 0 rpm, 30 1C 80.6 96.1 169 000 325 000 52

Comamonas sp. EB172 E 30 0.05 M NaOH, 1 h, 0 rpm, 4 1C 88.6 96.8 477 000 163 000 65

Comamonas sp. EB172 E 30 Water, 5 h, 0 rpm, 30 1C 48.1 93.5 477 000 854 000 54

a Weight average molecular weight, which was determined by GPC from lyophilized cells.
b Not determined.