A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS - LC-MS/MS in Proteomics: Methods and Applications - page 345

Intens.

+MS2(ETD 714.8->PTR), 1.3-26.6min #(26-434)

4

x10

K T I/L - 0.1

V

I/L + 0.1

F

A

1+

277.0

G

T - 0.1 I/L - 0.1

K - 0.1

0.8

T

0.6

E + 0.1

T + 0.1 I/L - 0.1

V

I/L + 0.1 225.9

D

2+

S + 0.1

1+

0.4 960.1

1136.7

1+

3+

390.2

2856.0

2+
903.1

846.5

2+

1347.2

1+

4+

V - 0.1

537.3

2+ 1+

1+ 2142.0

T + 0.2I/L - 0.2

0.2

1+ H N

720.4 1737.9 1921.1

I/L E + 0.1

T + 0.1 S E E

203.0

1+ V - 0.1

D

2+

I/L - 0.1

1414. k - 0.1

G

636.4

I/L

2+

Q

I/L

R

1517.8 2+

1+

2+

1800.9

1579.9

2+

1041.6

1+

2557.2

S - 0.1 I/L - 0.1

T 2693.4

R N

D

Y - 0.1

0.0

500 1000 1500 2000 2500 3000 m/z

Fig. 21.4. ETD/PTR with manual de novo sequence interpretation. ETD/PTR data generated from intact bovine ubiquitin ( A ) intact mass data.