Biomedical Engineering Reference
In-Depth Information
27. For quantification, the m / z for phospho-Aktide must be
extracted from the total chromatogram. The result will be
the extracted ion chromatogram (XIC), the elution profile
of which will provide us with the area proportional to the
studied peptide.
References
1. Manning, G., Plowman, G. D., Hunter, T.,
and Sudarsanam, S. (2002) Evolu-
tion of protein kinase signaling from
yeast to man. Trends Biochem Sci 27 ,
514-520.
2. Cohen, P. (2002) Protein kinases-the major
drug targets of the twenty-first century?, Nat
Rev Drug Discov 1 , 309-315.
3. Sawyers, C. L. (2008) The cancer biomarker
problem. Nature 452 , 548-552.
4. Haber, D. A., and Settleman, J. (2007) Can-
cer: drivers and passengers. Nature 446 ,
145-146.
5. Johnson, S. A., and Hunter, T. (2005)
Kinomics: methods for deciphering the
kinome. Nat Methods 2 , 17-25.
6. Turk, B. E. (2008) Understanding and
exploiting substrate recognition by pro-
tein kinases. Curr Opin Chem Biol 12 ,
4-10.
7. Martelli, A. M., Tazzari, P. L., Evange-
listi, C., Chiarini, F., Blalock, W. L., Billi,
A. M., Manzoli, L., McCubrey, J. A., and
Cocco, L. (2007) Targeting the phos-
phatidylinositol 3-kinase/Akt/mammalian
target of rapamycin module for acute myel-
ogenous leukemia therapy: from bench to
bedside. Curr Med Chem 14 , 2009-2023.
8. Fasolo, A., and Sessa, C. (2008) mTOR
inhibitors in the treatment of cancer. Expert
Opin. Investig. Drugs 17 , 1717-1734.
9. Vanhaesebroeck, B., Leevers, S. J., Ahmadi,
K., Timms, J., Katso, R., Driscoll, P. C.,
Woscholski, R., Parker, P. J., and Waterfield,
M. D. (2001) Synthesis and function of 3-
phosphorylated inositol
synthetic peptide RPRAATF allows specific
assay of Akt activity in cell
lysates. Anal.
Biochem. 305 , 32-39.
13. Shults, M. D., Janes, K. A., Lauffen-
burger, D. A., and Imperiali, B. (2005)
A multiplexed homogeneous fluorescence-
based assay for protein kinase activity in cell
lysates. Nat. Methods 2 , 277-283.
14. Hazeki, O., Hazeki, K., Katada, T., and
Ui, M. (1996) Inhibitory effect of wort-
mannin on phosphatidylinositol 3-kinase-
mediated cellular events. J. Lipid Mediat. Cell
Signal 14 , 259-261.
15. Vlahos, C. J., Matter, W. F., Hui, K. Y., and
Brown, R. F. (1994) A specific inhibitor
of phosphatidylinositol 3-kinase, 2-(4-
morpholinyl)-8-phenyl-4H-1-benzopyran-
4-one (LY294002). J. Biol. Chem. 269 ,
5241-5248.
16. Powis, G., Bonjouklian, R., Berggren, M.
M., Gallegos, A., Abraham, R., Ashendel,
C., Zalkow, L., Matter, W. F., Dodge,
J., Grindey, G., and et al. (1994) Wort-
mannin, a potent and selective inhibitor
of phosphatidylinositol-3-kinase. Cancer Res.
54 , 2419-2423.
17. Alessi, D. R., Caudwell, F. B., Andjelkovic,
M., Hemmings, B. A., and Cohen, P. (1996)
Molecular basis for the substrate specificity of
protein kinase B; comparison with MAPKAP
kinase-1 and p70 S6 kinase. FEBS Lett. 399 ,
333-338.
18. Kobayashi, T., Deak, M., Morrice, N., and
Cohen, P. (1999) Characterization of the
structure and regulation of two novel iso-
forms of serum- and glucocorticoid-induced
protein kinase. Biochem.
lipids. Annu. Rev.
Biochem. 70 , 535-602.
10. Yuan, T. L., and Cantley, L. C. (2008) PI3K
pathway alterations in cancer: variations on a
theme. Oncogene 27 , 5497-5510.
11. Cutillas, P. R., Khwaja, A., Graupera, M.,
Pearce, W., Gharbi, S., Waterfield, M., and
Vanhaesebroeck, B. (2006) Ultrasensitive
and absolute quantification of the phospho-
inositide 3-kinase/Akt signal transduction
pathwaybymassspectrometry. Proc. Natl.
Acad. Sci. USA 103 , 8959-8964.
12. Bozinovski, S., Cristiano, B. E., Marmy-
Conus, N., and Pearson, R. B. (2002) The
J. 344 Pt 1,
189-197.
19. Park, J., Leong, M. L., Buse, P., Maiyar, A.
C., Firestone, G. L., and Hemmings, B. A.
(1999) Serum and glucocorticoid-inducible
kinase (SGK) is a target of the PI 3-kinase-
stimulated signaling pathway. Embo. J. 18 ,
3024-3033.
20. Villen, J., and Gygi, S. P. (2008) The
SCX/IMAC enrichment approach for global
phosphorylation analysis by mass spectrome-
try. Nat. Protoc. 3 , 1630-1638.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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