Biomedical Engineering Reference
In-Depth Information
24. Labelled glycans can be stored at -20
◦
C in the dark.
25. DHB is the most commonly used matrix for MALDI-
TOF analysis of carbohydrates. Make sure to exclude high-
intensity matrix ions at low mass (
m
/
z
<850), which are
particularly abundant in NP-LC-MALDI.
26. MS/MS methods set up for the CID of peptides in
-
cyano-4-hydroxycinnamic acid (CHCA) will not give good
fragmentation.
27. Fragmentation of [M + H]
+
will give predominantly glyco-
sidic cleavages (B
n
and Y
n
ions) and little cross-ring frag-
ments.
α
Acknowledgements
This work was funded by the Food Standards Agency (for TT).
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