Analysis of Carbohydrates on Proteins by Offline Normal-Phase Liquid Chromatography MALDI-TOF/TOF-MS/MS - LC-MS/MS in Proteomics: Methods and Applications - page 147

1,5 X 3 HOH 2 C

O

HO

O

OH

HO

3,5 A 3

1,5 X 1

1,5 X 2

HOH 2 C HOH 2 C

H 2 C

Y 1

OH

O O

N -AA

HO O O

3,5 A 2

B 3

1,3 A 2

OH HO

(a)

HOH 2 C NHAc NHAc

C 2

O

1,5 X 1 β

HO O

H 3 C

1,5 X 3

1,5 X 3

O

% Intensity

OH

1066.4

HO

B 3

HO O

100

712.4

OH

HO

D 2

90

347.3

1,5 X 2

80

742.4

C 2 -2

1,5 X 1
3,5 A 3

70

[M + Na] +

525.4

1200.5

60

583.4

50

C 3

1,5 X 1 β

40

E 3

1082.4

655.4 30

Y 1

511.3

20

10

0

104.0 326.8 549.6 772.4 995.2 1218.0

Mass (m/z)

1,5 X 3

HOH 2 C

O

HO

O

OH

HO

3,5 A 3

1,5 X 2

1,5 X 1

HOH 2 C HOH 2 C

(b)

H 2 C

Y 1

O O OH

N -AA

HO O O

3,5 A 2

B 3

% Intens i ty

HO OH HO

NHAc

NHAc

C 2

1,5 X 1 β 100

H 3 C

O

90 O

HO

[M + Na] +

HO OH

80

3,5 A 3

1038.4

B 3

C 2

70

421.4

550.4

C 2 -2
D 2

365.3
363.3

60

1,5 X 2

1,5 X 1
E 3

742.4

50

347.3

539.4
493.4

C 3

40

568.4

1,5 X 3
1,5 X 1 β

Y 2 30

714.4

3,5 A 2

904.4

20

259.3

920.4

10

0

69.0 274.4 479.8 685.2 890.6 1096.0

Mass (m/z)

Fig. 8.3. MALDI-CID spectrum of (a) Man 3 GlcNAc 2 Fuc and (b) Man 2 GlcNAc 2 Fuc
labelled with 2-aminobenzoic acid (2-AA).