Biology Reference
In-Depth Information
Table 1.1 High-throughput platforms and mass spectrometry
instrumentation in systems biology
Offline
Separation of separation
proteins
a
of peptides
b
Platform
MS
References
Proteome
MudPIT
IT
26-28,33,43,
45,57,59,60,67,
68,71,76-78,
80,120,122
LC-ESI-MS/MS
Q-TOF
91,92
LC-ESI-MS/MS
IT
37,72
followed by
LC-ESI-MS (AMT)
FTICR
LC-ESI-MS/MS
IT
81
SCX, avidin
LC-ESI-MS/MS
IT
74,75,89
affinity (ICAT)
CE
LC-MS/MS
IT
40
Phosphorylated Proteins
LC-ESI(
−
)-MS
TQ
39
followed by
LC-ESI(
+
)-MS/MS
IMAC
LC-ESI-MS/MS
IT
97
IMAC,
LC-ESI-MS/MS
Q-TOF
103
SAX
Avidin affinity
LC-ESI-MS/MS
IT, Q-TOF
104
LC-ESI-MS/MS
IT
56
SDS-Page
IMAC
MALDI-MS
MALDI-TOF
103
followed by
Q-TOF
LC-ESI-MS/MS
MALDI-
MALDI-MS/MS
Q-TOF
SDS-Page
SCX
LC- ESI-MS/MS
IT
98
IP
IMAC
LC-ESI-MS/MS
IT
99
IMAC
LC-ESI-MS/MS
IT
83
SDS-Page
LC-ESI-MS,
IT
53,54
LC-ESI-MS/MS
IMAC, SCX
LC-ESI-MS/MS
IT
119
MALDI-MS/MS
MALDI-IT
62,63
Other Posttranslational Modifications of Proteins
MudPIT
IT
28,80,106
RP,RP
LC-MS/MS
Q-TOF
38
Lectin
AE, lectin
LC-MS/MS
Q-TOF
108
affinity
affinity
IP
SCX
LC-ESI-MS/MS
IT
107
SCX/avidin
LC-ESI-MS
n
LIT
110
affinity
22
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