Biology Reference
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Figure 1.3 Data-dependent analysis of a standard protein mixture digested
with three different proteases (illustration adapted from ref. [16] with data
from authors' laboratory). The panel presents a time window of 10 minutes of
an LC-MS/MS analysis (a). The chromatogram has a jagged appearance as
the instrument alternates between recording a full scan MS and three
consecutive MS/MS scans. Full scan MS spectrum at retention time 73.32 (on
chromatography experiments a minute is divided into 100 equal seconds) is
recorded as scan # 2761 (b). A range of 1000 m / z units is surveyed, from m / z
400 to m / z 1400. The three most abundant ions are selected for further MS/MS
analysis. Once an ion has been selected for MS/MS fragmentation, it is
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