Biology Reference
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Figure 7.10
Simplified metabolic reaction network of engineered
E. coli
for the
production of PHB. Bold arrows indicate three heterologous reactions that are
newly introduced into
E. coli
.
First, in the case of PHB accumulation up to 49% of dry cell weight
[96], it was predicted that about 24% of total carbon flux was directed
to the PHB pathway, and most of it was supplied through the
Entner-Doudoroff (ED) pathway (figure 7.11a). The flux of acetyl-CoA
into the TCA cycle, which competes with the PHB biosynthesis
pathway, decreased significantly. Next, the intracellular flux distribu-
tion in recombinant
E. coli
that accumulated PHB up to 78% of dry cell
weight was simulated [97] and compared with that predicted in a
non-PHB-producing strain (figure 7.11b). A significant amount of carbon
flux (74%) was directed into the PHB biosynthetic pathway, and most
of the other intracellular fluxes were severely reduced. Again, it was
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