Biology Reference
In-Depth Information
Table 5.3 ( Continued )
Name
Description
Advantages
Disadvantages
References
Zigmund
Provides a measure of the migration
Because a glass slide is used, it is
The assessment of
[67,69]
chamber
characteristics of cell populations.
possible to view the cells during the assay
migrated cells is
Like the Boyden chamber, a gradient
as they migrate across the bridge in time,
laborious and subjective.
is formed by a chemoattractant
unlike in the Boyden microchamber.
High-throughput
diffusing between two
The physical separation between the
chemotaxis assays
compartments. In this case,
wells increases the stability of the
using cell lines have not
however, the compartments are
chemokine gradient as compared
yet been developed.
represented by two rectangular
to the Boyden chamber.
wells that are cut into a Plexiglas
slide and separated by a narrow
bridge. The wells are filled with
medium, one of which contains
the chemoattractant, cells are
added, and a gradient forms as
the chemokine diffuses across
the bridge.
 
Search WWH ::




Custom Search