Mapping the Genotype–Phenotype Relationship in Cellular Signaling Networks: Building Bridges Over the Unknown - Systems Biology: Networks, Models, and Applications - page 154

Table 5.3 ( Continued )

Name Description Advantages Disadvantages References

Dunn Provides a measure of the migration Offers the best means to view the cells The assessment of [67,69,70]

chemotaxis characteristics of populations of during the chemotaxis assay: a glass the number of

chamber cells. Like the Boyden and Zigmund slide is used, enabling microscopy, and migrating cells is

microchambers, a gradient is formed the wells are cut precisely into the glass laborious and

by a chemoattractant diffusing slide, ensuring better transmission subjective.

between two compartments. In this properties for many different types of High-throughput

design, however, the wells are light microscopy. chemotaxis assays

arranged in a concentric layout. The use of a concentric layout maximizes have not yet been

Additionally, the wells are precisely the stability of the chemokine gradient; developed.

cut into a glass slide, which the edge effects associated with the

enables better transmission Boyden chamber and the linear bridge

properties for light microscopy. in the Zigmund chamber are avoided.

Flow cytometry Allows large numbers of cells to be Highly sensitive and quantitative, Severely limited in [71]

analyzed rapidly and automatically i.e., capable of precise, discrete its ability to handle

for physiological characteristics. measurements of fluorescent signals. multiple discrete

Cells are stained with fluorescent Enables the simultaneous quantitative samples of cells,

dyes, suspended in solution, analysis of multiple optical markers. though recent

and passed in a stream before a variations of flow

laser or other light source. The cytometry, including

subsequent measurements that plug flow cytometry,

categorize the cells are based on have attempted to

how the light-sensitive dye reacts solve this problem.

to the light source. This technique Informatic systems,

offers real-time, quantitative i.e., tools for accurately

measurements of characteristics tracking data, have yet